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Lab Reference · Reconstitution

How to reconstitute peptides, without guessing at the volume.

Everything that happens after the courier leaves: which solvent belongs in which vial, the arithmetic that turns milligrams per vial into units on a syringe, a per-vial volume reference for the catalog, and how long the solution actually lasts once the cake is gone. Laboratory reference only — no administration guidance, no dosing.

27Compounds in the volume table
0.9%Benzyl alcohol in bacteriostatic water
28 dMulti-use window at +4 °C
10 µLOne unit on a U-100 syringe
Quick Answer

In one paragraph: To reconstitute a lyophilized peptide, let the vial and the solvent reach room temperature, wipe both septa with 70% isopropyl alcohol, draw a measured volume of bacteriostatic water into a sterile single-use syringe, let it run slowly down the inner wall of the peptide vial rather than onto the cake, then swirl — never shake — until the solution is optically clear. The volume you choose sets the concentration, not the amount of peptide: 5 mg in 2 mL is 2.5 mg/mL, which on a U-100 syringe means 25 µg per unit. Store the reconstituted vial upright at +4 °C and use it inside 14 to 28 days depending on the sequence, or aliquot it into single-use volumes and freeze at −20 °C.

  • Default solvent: bacteriostatic water — sterile water for injection with 0.9% benzyl alcohol, which is what permits repeated access to the same vial.
  • The only equation: concentration = mass in the vial ÷ solvent volume added. Nothing else in this page changes that.
  • Unit conversion: a U-100 syringe puts 100 units in 1 mL, so one unit is 0.01 mL, or 10 µL.
  • Storage after reconstitution: 14–28 days at +4 °C; aliquot and freeze for anything longer, and never refreeze a thawed aliquot.
  • Legal frame: research-grade compounds for in-vitro laboratory work only, not for human or veterinary use.
First principles

What reconstitution actually is.

A peptide does not arrive as a powder because powder is convenient. It arrives that way because water is the fastest route to hydrolysis, deamidation and aggregation, and freeze-drying removes it. Reconstitution is the deliberate, controlled reversal of that step — and the point at which a lot that cleared every release assay becomes your responsibility.

The cake

Freeze-drying removes water by sublimation under vacuum and leaves a porous solid: the lyophilized cake. A correct cake is uniform and white, occupies roughly the volume the solution did, and shows no collapse, no shrinkage away from the glass and no crystalline glitter. Its appearance is the first piece of quality information you get, and it is free.

The vacuum

The vial is sealed under partial vacuum at the end of the lyophilisation cycle. That negative pressure is why solvent is drawn in almost on its own when the needle enters, and why a vial that offers no resistance at all should be treated with suspicion — a seal that failed in transit means the cake has been exposed to atmospheric moisture.

The solvent

The liquid you add does three jobs at once: it dissolves the cake, it fixes the concentration for the rest of the vial's life, and it either does or does not protect the contents from the microorganisms your needle introduces on every subsequent entry. Only one of those three is chemistry. The other two are bookkeeping and hygiene, and they are where most mistakes happen.

Solvent selection

Bacteriostatic water, WFI, or acid.

Four liquids come up in practice. Three of them are correct in specific circumstances and one of them is almost never what you want. The deciding question is not solubility — most of this catalog dissolves in plain water — but how many times the septum will be pierced, and whether a preservative would interfere with what you are measuring.

Bacteriostatic water — the default

CompositionSterile WFI + 0.9% benzyl alcohol
Multi-use windowUp to 28 days after first puncture
Storage after opening+4 °C
Use it whenThe vial will be accessed more than once
Avoid it whenBenzyl alcohol would confound the assay
Catalog item20 mL vial, USD 14

Sterile water for injection

CompositionWater, preservative-free
Multi-use windowNone — single session
Storage after openingDiscard the remainder
Use it whenWhole vial is consumed or aliquoted at once
Avoid it whenRepeated entry over days is planned
Typical roleCell-culture and analytical work

0.6% acetic acid

CompositionDilute acetic acid in sterile water
PurposeSolubilising sequences that resist neutral pH
Typical caseIGF-1 LR3
MethodDissolve in acid first, dilute after
PreservativeNone — treat as single-session
CautionNot interchangeable with bac water

0.9% saline — the wrong default

CompositionSodium chloride 0.9% in water
PreservativeUsually none
ProblemIonic strength can promote aggregation
Multi-use windowNone in the plain formulation
Use it whenA protocol specifically calls for it
OtherwisePrefer bacteriostatic water

The practical rule is short. If you are going to open the vial once, preservative-free water is fine and arguably cleaner. If you are going to open it on Monday and again on Thursday, the benzyl alcohol in bacteriostatic water is doing work that nothing else in the workflow does. The one solvent question that is genuinely compound-specific is solubility at neutral pH, and in this catalog that affects exactly one entry — IGF-1 LR3. Everything else dissolves in water.

The arithmetic

Milligrams in the vial, units on the syringe.

This is the part people get wrong, and it is the part that is genuinely trivial once written down. There are two equations and one unit conversion. Nothing on this page is more complicated than that.

The two equations

ConcentrationC = m ÷ V
Volume to drawV = m ÷ C
mMass printed on the vial (mg)
VSolvent volume added (mL)
CResulting concentration (mg/mL)
Invariantm never changes. Only C does.

U-100 graduation

1 mL100 units
1 unit0.01 mL = 10 µL
10 units0.10 mL = 100 µL
25 units0.25 mL
50 units0.50 mL
Kit barrel0.5 mL = 50 units full scale
Worked examples

Three vials, three concentrations.

The same three lines of arithmetic applied to a 5 mg vial, a 1 mg vial and a 10 mg vial. Note that the smallest vial produces the lowest concentration and therefore the most syringe units per milligram — small vials are easier to measure, not harder.

BPC-157

5 mg / vial
A

Five milligrams of lyophilized peptide dissolved in 2 mL of bacteriostatic water gives a 2.5 mg/mL solution, which is the most common working concentration in this catalog because it lands on a round 25 µg per syringe unit.

Mass in vial
5 mg
Solvent added
2.0 mL
Concentration
2.5 mg/mL
Per U-100 unit
25 µg
The full 0.5 mL barrel of the kit syringe holds 50 units of this solution, or 1.25 mg of peptide — one quarter of the vial.

IGF-1 LR3

1 mg / vial
B

One milligram in 1 mL gives 1 mg/mL and 10 µg per unit — the finest resolution available on a standard insulin syringe, which matters because this is the lowest-mass vial in the catalog.

Mass in vial
1 mg
Solvent added
1.0 mL
Concentration
1 mg/mL
Per U-100 unit
10 µg
This is the one entry in the catalog where the solvent is not bacteriostatic water by default: dissolve in 0.6% acetic acid first, then dilute if the protocol requires it.

3-GLP (Retatrutide)

10 mg / vial
C

Ten milligrams in 2 mL gives 5 mg/mL and 50 µg per unit. Doubling the mass at constant volume doubles the concentration and halves the number of units needed for the same quantity of peptide.

Mass in vial
10 mg
Solvent added
2.0 mL
Concentration
5 mg/mL
Per U-100 unit
50 µg
Acylated, albumin-binding peptides foam more readily than short unmodified sequences. Deliver the solvent slowly down the wall and give this one the full two minutes to stand.
Reference table

Reconstitution volumes for the catalog.

Every catalog item that ships as a lyophilized cake, with the mass on the vial, the solvent this reference standardises on, a working volume chosen so the concentration lands on a round figure, and the mass carried by one unit of a U-100 syringe. The volume column is a convention, not a specification: any volume is chemically valid, and changing it changes only the concentration column.

Compound Category Per vial Solvent Volume Concentration Per unit (10 µL)
IGF-1 LR3 1 mg / vial Growth & Anabolic 1 mg 0.6% AcOH 1 mL 1 mg/mL 10 µg
CJC-1295 (No DAC) 5 mg / vial Growth & Anabolic 5 mg BAC 2 mL 2.5 mg/mL 25 µg
CJC-1295 (with DAC) 5 mg / vial Growth & Anabolic 5 mg BAC 2 mL 2.5 mg/mL 25 µg
CJC-1295 No DAC + Ipamorelin Blend 5 mg + 5 mg / vial Growth & Anabolic 10 mg BAC 2 mL 5 mg/mL 50 µg
Ipamorelin 5 mg / vial Growth & Anabolic 5 mg BAC 2 mL 2.5 mg/mL 25 µg
Tesamorelin 5 mg / vial Growth & Anabolic 5 mg BAC 2 mL 2.5 mg/mL 25 µg
MOTS-c 10 mg / vial Growth & Anabolic 10 mg BAC 2 mL 5 mg/mL 50 µg
BPC-157 5 mg / vial Healing & Recovery 5 mg BAC 2 mL 2.5 mg/mL 25 µg
TB-500 (Thymosin β-4 Fragment) 5 mg / vial Healing & Recovery 5 mg BAC 2 mL 2.5 mg/mL 25 µg
BPC-157 + TB-500 Blend 5 mg + 5 mg / vial Healing & Recovery 10 mg BAC 2 mL 5 mg/mL 50 µg
GHK-Cu (Copper Peptide) 50 mg 50 mg / vial Healing & Recovery 50 mg BAC 5 mL 10 mg/mL 100 µg
GHK-Cu (Copper Peptide) 100 mg 100 mg / vial Healing & Recovery 100 mg BAC 10 mL 10 mg/mL 100 µg
3-GLP (Retatrutide) 10 mg / vial Metabolic & Weight 10 mg BAC 2 mL 5 mg/mL 50 µg
AOD-9604 5 mg / vial Metabolic & Weight 5 mg BAC 2 mL 2.5 mg/mL 25 µg
Melanotan II (MT-2) 10 mg / vial Skin & Cosmetic 10 mg BAC 2 mL 5 mg/mL 50 µg
Melanotan I (MT-1, Afamelanotide) 10 mg / vial Skin & Cosmetic 10 mg BAC 2 mL 5 mg/mL 50 µg
GLOW 70 mg Blend 70 mg total (50 + 10 + 10) Skin & Cosmetic 70 mg BAC 5 mL 14 mg/mL 140 µg
KLOW 80 mg Blend 80 mg total (50 + 10 + 10 + 10) Skin & Cosmetic 80 mg BAC 5 mL 16 mg/mL 160 µg
SNAP-8 10 mg / vial Skin & Cosmetic 10 mg BAC 2 mL 5 mg/mL 50 µg
Selank 5 mg / vial Cognitive & Sleep 5 mg BAC 2 mL 2.5 mg/mL 25 µg
Semax 5 mg / vial Cognitive & Sleep 5 mg BAC 2 mL 2.5 mg/mL 25 µg
DSIP (Delta Sleep-Inducing Peptide) 5 mg / vial Cognitive & Sleep 5 mg BAC 2 mL 2.5 mg/mL 25 µg
PT-141 (Bremelanotide) 10 mg / vial Sexual & Hormonal 10 mg BAC 2 mL 5 mg/mL 50 µg
HCG (Human Chorionic Gonadotropin) 5 000 IU / vial Sexual & Hormonal 5 000 IU BAC 5 mL 1 000 IU/mL 10 IU
Teriparatide (PTH 1-34) 10 mg / vial Sexual & Hormonal 10 mg BAC 2 mL 5 mg/mL 50 µg
NAD+ (Nicotinamide Adenine Dinucleotide) 500 mg / vial Support & Solvents 500 mg BAC 10 mL 50 mg/mL 500 µg
L-Glutathione (Reduced) 1 500 mg / vial Support & Solvents 1 500 mg WFI 10 mL 150 mg/mL 1.5 mg

BAC = bacteriostatic water · WFI = sterile water for injection, preservative-free · 0.6% AcOH = dilute acetic acid. The concentration and per-unit columns are computed from the two preceding columns at page render, so they cannot drift out of step with the catalog. The remaining 7 items in the 34-compound catalog do not appear here because they need no solvent: three ship as capsules or bulk powder, two are supplied pre-dissolved as 30 mL topical solutions, and two are the support items used to perform the reconstitution itself.

Bench protocol

How to reconstitute peptides in eight steps.

The order matters more than the speed. Steps two and three are the ones habitually skipped, and they are the two that determine whether the rest of the vial is trustworthy a week later.

  1. 01

    Equilibrate to room temperature

    Take the peptide vial and the solvent out of refrigeration and leave them on the bench for ten to fifteen minutes. Cold solvent wets a lyophilized cake more slowly, and a cold vial pulled straight into a warm room condenses moisture on the glass exactly where you are about to work.

    ≈ 10–15 min · passive
  2. 02

    Do the arithmetic first

    Divide the mass printed on the vial by the volume you intend to add. Write the result on the label before anything is opened. A concentration reconstructed from memory three days later is a guess, and a guess in this position propagates into every measurement that follows.

    ≈ 1 min · C = m ÷ V
  3. 03

    Disinfect both septa

    Wipe the rubber septum of the peptide vial and of the solvent vial with a fresh 70% isopropyl alcohol pad, one pad per septum, and let each air-dry for roughly thirty seconds. Alcohol kills by evaporating; a septum that is still wet has not finished being disinfected, and the residue rides the needle inward.

    ≈ 1 min · 70% IPA, air-dry
  4. 04

    Draw the measured volume

    With a sterile single-use syringe, draw the solvent volume you calculated in step two. Read the graduation at eye level against the leading edge of the plunger seal, not the top of the flange, and clear air bubbles before withdrawing from the solvent vial.

    ≈ 1 min · single-use syringe
  5. 05

    Deliver down the vial wall

    Enter the peptide septum at a shallow angle and let the stream run down the inside of the glass. Do not aim it at the cake. The residual vacuum will pull the liquid in faster than you intend, so resist the plunger rather than pushing it — a jet directly into the cake is the most common cause of foaming.

    ≈ 30 s · resist the plunger
  6. 06

    Stand, then swirl — never shake

    Withdraw the needle, drop the syringe straight into a sharps container, and let the vial stand upright for one to two minutes. Then roll or swirl it gently between the fingers until the solution is uniform. Shaking whips air into the liquid and shears longer sequences at the air-liquid interface.

    ≈ 2 min · gentle swirl
  7. 07

    Inspect against two backgrounds

    Hold the vial against white, then against black. White reveals particulates and fibres; black reveals haze and turbidity. A correctly reconstituted solution is optically clear against both. If it is not, stop here — the vial has told you something that no further handling will fix.

    ≈ 30 s · white then black
  8. 08

    Label, date, refrigerate

    Write the concentration and the reconstitution date directly on the vial. Store it upright at +4 °C away from light. If it will not be consumed inside the stability window below, split it into single-use aliquots now, while the solution is still fresh and the vial has been entered exactly once.

    ≈ 2 min · +4 °C, upright
Sterile technique

The septum is the only barrier you have.

Every assay in the release workflow — HPLC purity, mass-spectrometric identity, endotoxin burden — describes the contents of a sealed vial. None of them survives contact with a used needle. The point of sterile technique is not ritual; it is that the analytical work already paid for stops being meaningful the moment the barrier is breached carelessly.

One pad, one septum, one direction

A 70% isopropyl pad is a single-use item. Wiping two septa with the same pad transfers whatever was on the first to the second. Wipe once, in one direction, and discard. The IGF1 Shop injection kit ships 100 individually wrapped pads against 50 syringes precisely because the correct ratio is two pads per session.

Never re-enter with a used needle

A needle that has been through a septum once is no longer sterile, and it is also blunter — coring pushes fragments of rubber into the vial. The kit syringes are 29-gauge with permanently attached needles, single-use and EO-sterilised. There is no procedure in which reusing one is the right call.

Sharps go in a sharps container

Used syringes belong in a rigid, puncture-resistant container, disposed of according to local rules for medical sharps. This is the one step in the protocol that protects somebody other than you, and it is not supplied in any kit — you have to have arranged it before you start.

Troubleshooting

What the vial is telling you.

Most reconstitution problems announce themselves visually within two minutes. The table below maps what you can see to the most probable cause and to whether the material is still usable. When a row says stop, it means stop: no amount of warming, swirling or waiting recovers an aggregated peptide.

What you observeMost likely causeWhenAction
Dense foam on the surface Solvent jetted onto the cake, or the vial was shaken Immediate Let it settle, then swirl
Cake still visible after five minutes Cold solvent, or a sequence that needs an acidic diluent Immediate Warm to room temp, re-swirl
Persistent haze against a black background Aggregation — shear, agitation or wrong ionic strength Immediate Stop, do not use
Fibres or floating specks against white Particulate contamination or rubber coring Immediate Stop, photograph, report
Cake collapsed or shrunken on arrival Lyophilisation cycle or vacuum seal failure Before solvent Do not reconstitute, report
No resistance when the needle enters Vacuum lost in transit Before solvent Inspect the cake first
Precipitate forming after refrigeration Solubility limit exceeded at +4 °C Hours to days Reconstitute more dilute next time
Deep blue solution Copper(II) chelate of GHK-Cu — expected Immediate Normal, proceed
Hard stops

Four things that end the vial.

These are not preferences. Each one either destroys the material or destroys your ability to say what is in it, and neither is recoverable by careful handling afterwards.

F1

Shaking a reconstituted vial

Mechanical agitation denatures peptides at the air-liquid interface and produces aggregates that will not redissolve. The damage is invisible until it is haze, and by then the sequence has already been altered. Swirl, stand, wait — the cake dissolves on its own timescale.

F2

Repeated freeze-thaw cycling

Freezing itself is well tolerated. The cycle is not: each thaw concentrates solutes at the receding ice boundary and subjects the peptide to a local environment nothing like the bulk solution. Aliquot before freezing, thaw once, and discard what is left over.

F3

Re-entering with a used needle

One pass through a septum ends the sterility of the needle and blunts it enough to core the rubber. A vial re-entered with a used needle carries an unknown microbial and particulate load, which makes every analytical figure on its Certificate of Analysis inapplicable to what is now inside.

F4

An unlabelled vial

A refrigerated vial with no concentration and no date written on it is unusable material, whatever is dissolved in it. There is no way to reconstruct either number from the vial itself, and estimating them turns a measured experiment into an anecdote.

Compound-specific notes

Where the general rule needs an exception.

Most of the catalog behaves identically: bacteriostatic water, gentle swirl, clear solution. Six cases do not, and five of the six are predictable from the chemistry printed on the product page.

IGF-1 LR3 and acidic diluents

An 83-residue analogue is a different solubility problem from a five-residue peptide. IGF-1 LR3 dissolves reluctantly at neutral pH; 0.6% acetic acid is the conventional first solvent, with dilution afterwards if the protocol calls for it. Forcing it into plain water with agitation is the fastest way to aggregate it.

GHK-Cu and the blue solution

The copper(II) chelate absorbs in the red, so a correctly reconstituted GHK-Cu solution is deep blue. This is the single case in the catalog where colour is not a warning sign. The 50 mg and 100 mg vials are the same compound at different fill masses, and both take the same 10 mg/mL working concentration.

Co-lyophilised blends

Four catalog entries are blends freeze-dried together in one vial. One volume of solvent reconstitutes every component simultaneously, and the concentration column in the table above refers to the combined mass. The individual component masses are printed on the product page for each blend.

High-mass support compounds

NAD+ at 500 mg and L-glutathione at 1 500 mg per vial carry far more solid than any peptide in the catalog. Both take 10 mL so the solution stays well clear of saturation, and glutathione oxidises on contact with air — reconstitute it immediately before use rather than storing the solution.

HCG is measured in IU

A glycoprotein hormone is standardised by biological activity, not by mass, so the vial reads 5 000 IU rather than a number of milligrams. The arithmetic is unchanged — 5 000 IU in 5 mL is 1 000 IU/mL — but every figure downstream carries IU as its unit, and mixing the two conventions in one notebook is a common and expensive error.

Topicals arrive already in solution

The two 30 mL topical compounds in the catalog are supplied pre-dissolved in an ethanol and propylene glycol vehicle at a stated concentration. They need no reconstitution and must not be diluted with water, which would drop the solute out of the vehicle. They are absent from the volume table for that reason.

After the cake is gone

Post-reconstitution stability.

A reconstituted peptide is a decaying asset, and the rate depends on three things you control: temperature, the presence of a preservative, and how many times the vial is opened. The windows below are the ones IGF1 Shop publishes on its quality documentation.

Room temperature
PreservativeIrrelevant
StatusNot a storage state

Ambient temperature is a working condition for the ten minutes the vial is on the bench, not a place to leave a solution overnight.

14 dWFI, +4 °C
PreservativeNone
EntriesMinimise

Lower end of the published 14–28 day range. Preservative-free solvent gives no protection against what the needle brings in.

28 dBAC water, +4 °C
Preservative0.9% benzyl alcohol
EntriesRepeated, permitted

The upper end of the published window, and the reason bacteriostatic water is the default solvent for any multi-session vial.

−20 °CAliquoted
FormatSingle-use volumes
Thaw cyclesExactly one

The long-term option, and the only one that survives past the refrigerated window. −80 °C where available; never refreeze a thawed aliquot.

Before reconstitution — sealed lyophilized vial

Ambient, darkSeveral weeks
+4 °C12 months
−20 °C24 months
Shipping formAmbient, insulated mailer
LightKeep in the carton
Printed onPer-lot Certificate of Analysis

After reconstitution — solution

Bacteriostatic water, +4 °CUp to 28 days
Preservative-free, +4 °CShorter — 14 days upper bound
Frozen aliquots−20 °C or −80 °C
Freeze-thaw cycles1
OrientationUpright, away from light
Label must carryConcentration + date

The storage profile for the specific lot you received is printed on its Certificate of Analysis, alongside the batch number, the release date and the three release assays. Where the COA and a general reference disagree, the COA wins: it describes your vial, this page describes the class. The reasoning behind the ambient-temperature shipping decision is set out in the shipping policy.

What the assays do and do not cover

Where the COA stops and you begin.

Three independent assays stand behind every released lot, and all three describe the contents of a sealed vial at the moment of release. Understanding what each one can and cannot predict about what you see on the bench is the difference between reading a COA and quoting one.

HPLC tells you what fraction is the compound

Reverse-phase HPLC at 220 nm against a reference standard reports area-percent purity, with a release floor of 99.0%. It says how much of the solid is the intended sequence. It says nothing about how that solid will behave in your chosen solvent — a 99.6% lot and a 99.1% lot dissolve identically. See the HPLC method for the full parameter set.

Mass spectrometry confirms it is the right molecule

ESI-MS confirms molecular weight to within ±1 Da of theoretical, catching truncations and oxidation that co-elute invisibly under an HPLC peak. It is an identity check, not a handling prediction. The method is documented on the mass spectrometry page.

Endotoxin is the one your technique can undo

The kinetic chromogenic LAL assay caps released lots below 0.5 EU/mg. That figure describes a sealed vial. Endotoxin introduced afterwards — by a re-used needle, a wet septum, a non-sterile solvent — is invisible, cumulative and entirely yours. The assay is explained on the endotoxin testing page.

All three assays are run by a contract laboratory that is structurally independent of the synthesis facility and accredited under ISO/IEC 17025:2017. The end-to-end release sequence is described on the quality workflow page, and the terminology used throughout — lyophilisation, lyophilized cake, bacteriostatic water, endotoxin unit — is defined in the glossary.

Checklist

On the bench before you start.

Five items. Two of them come from the catalog, two are consumables you should already have, and one is a decision rather than an object.

Supplied by IGF1 Shop

The compoundSealed lyophilized vial
Certificate of AnalysisPer lot, in the shipment
Bacteriostatic water20 mL vial, ordered separately
Injection kit50 syringes + 100 pads
Syringe formatU-100, 0.5 mL, 29G, single-use
Pads70% isopropyl, individually wrapped

Supplied by you

Sharps containerRigid, puncture-resistant
Refrigerator+4 °C, stable
Freezer−20 °C for aliquots
Label and markerSolvent-resistant ink
The concentration decisionMade before opening
Legal complianceYour jurisdiction, your responsibility

Both catalog items sit in the Support & Solvents category: bacteriostatic water at USD 14 for a 20 mL vial, and the injection kit at USD 25 for 50 sterile U-100 syringes and 100 alcohol prep pads. One 20 mL vial of solvent reconstitutes ten 2 mL vials, so the ratio that usually makes sense is one solvent vial per handful of compounds.

Scope of this page. This is a laboratory handling reference: solvent selection, concentration arithmetic, sterile technique and storage. It contains no dosing guidance and no administration protocol, and it is not one. IGF1 Shop supplies research-grade compounds for in-vitro laboratory work only, not for human or veterinary use; several catalog compounds are investigational drugs that are not approved for human use outside trial protocols. Legality of import, possession and use varies by jurisdiction and is the buyer's responsibility — the full terms are set out in the research disclaimer and the terms of use.

FAQ

Questions about how to reconstitute peptides.

How much bacteriostatic water do I add to a 5 mg vial of BPC-157?
This reference standardises on 2 mL of bacteriostatic water for a 5 mg vial, giving 2.5 mg/mL. On a U-100 insulin syringe one unit is 0.01 mL, so one unit carries 25 µg of peptide and the full 0.5 mL barrel carries 1.25 mg. Any volume works chemically — 1 mL gives 5 mg/mL, 5 mL gives 1 mg/mL — because the volume changes the concentration, never the total mass of peptide in the vial. Pick the volume that makes your arithmetic round.
What is the difference between bacteriostatic water and sterile water for injection?
Sterile water for injection (WFI) is preservative-free: once the septum is pierced the vial has no defence against microbial ingress, so it is a single-session solvent. Bacteriostatic water is the same water with 0.9% benzyl alcohol added as a bacteriostatic preservative, which is what allows repeated access to the same vial for up to 28 days at +4 °C. Use WFI when the preservative would interfere with the assay or with a sensitive sequence; use bacteriostatic water for anything you will access more than once.
How long does a reconstituted peptide last in the fridge?
IGF1 Shop states 14 to 28 days at +4 °C for a reconstituted solution, depending on the sequence, with the 28-day figure tied to the bacteriostatic preservative in the solvent. Preservative-free water shortens that window considerably. For anything longer, aliquot into single-use volumes and freeze at −20 °C or −80 °C rather than repeatedly re-entering one refrigerated vial.
How do I convert mg/mL into insulin-syringe units?
A U-100 syringe is graduated so that 100 units equal 1 mL; one unit is therefore 0.01 mL, or 10 µL. Multiply the concentration in mg/mL by 0.01 to get the mass carried by one unit. A 2.5 mg/mL solution carries 25 µg per unit; a 5 mg/mL solution carries 50 µg per unit. Note that the 0.5 mL barrel supplied in the IGF1 Shop injection kit holds 50 of those units, not 100 — the 100-unit graduation belongs to the 1 mL barrel.
Can I shake the vial to make the powder dissolve faster?
No. Shaking drives air into the solution, generates foam at the air-liquid interface, and mechanically shears longer sequences. Deliver the solvent slowly down the inner wall of the vial rather than onto the cake, then let the vial stand for a minute or two and swirl it gently. Most peptides in this catalog dissolve without any agitation at all once the solvent has wetted the cake.
My reconstituted solution is cloudy or has visible particles — what now?
Stop and do not use it. A correctly reconstituted peptide solution is optically clear. Persistent haze, floating fibres or sediment that will not redissolve at room temperature indicate aggregation, incomplete dissolution or contamination. Photograph the vial, note the lot number etched on the cap and printed on the Certificate of Analysis, and contact support. One exception: GHK-Cu produces a deep blue solution because of the copper(II) chelate — that colour is the compound, not a defect.
Can I freeze a reconstituted peptide?
Yes, provided you aliquot first. The damage in frozen storage does not come from the cold, it comes from the freeze-thaw cycle: each thaw concentrates solutes at the ice boundary and stresses the peptide. Split the reconstituted solution into single-use volumes immediately after reconstitution, freeze those at −20 °C or −80 °C, and thaw each one once. Never refreeze a thawed aliquot.
Does the reconstitution volume change how much peptide I have?
No. The vial contains the mass printed on the label and on the Certificate of Analysis regardless of what you add to it. Adding 1 mL rather than 2 mL to a 5 mg vial produces 5 mg/mL instead of 2.5 mg/mL — twice the concentration, the same 5 mg of peptide. Volume is a unit-conversion decision, not a potency decision.

Still unresolved? Verified orders can reach customer support with the lot number from the vial cap. For a broader comparison of what is in the catalog and why, see the 2026 buyer's guide.